單細胞與空間組學中心實驗室

如需服務、技術諮詢或項目討論，請聯繫 Joaquim Vong 博士（郵箱： joaquim@cuhk.edu.hk / 39430318）。

如需服務，請填寫我們的樣品提交表格。

服務費用包含：（請諮詢）

✓ 套餐：從細胞到測序文庫
✓ 細胞質量和活力檢查/基礎質控
✓ 測序文庫質控和定量分析
✓ 專家建議（幹/溼實驗室）
✓ 實驗問題排除

× 不含測序服務
× 重複初始樣品質控檢查可能會產生額外費用

A. 測序文庫
B. cDNA 和測序文庫的質量控制報告
C. 索引信息
最終產物是一個雙鏈 cDNA 分子，帶有測序接頭和 3 個不同的索引（10X 條形碼：細胞信息；UMI：轉錄本信息；樣本索引：樣本信息）。測序文庫的質量通過 Agilent 4200 TapeStation 系統進行評估。

來源：10X Genomics

用戶提交樣本前，需填寫並簽署“單細胞與空間組學中心實驗室服務申請表”

https://webapp.sbs.cuhk.edu.hk/eform/view.php?id=55400

所有用戶必須聲明提交給單細胞與空間組學中心實驗室的樣本不含任何生物危害物質。

爲了生成最佳質量的數據，我們將進行細胞質量初步評估。如果初始細胞質量檢查未通過，用戶可能面臨數據質量不佳的風險。

預訂服務後，已確認的服務申請表副本將轉發給最終用戶和相應的 PI。

建議根據樣本性質進行初步樣本提交評估（樣本質量、數量等），以優化樣本質量。提交樣本前會安排可能的實驗討論。

整個工作流程大約需要 2 個工作日。由於每週可處理的樣本數量有限，建議用戶在提交前優化樣本質量。服務預訂將遵循先到先得的原則。

由於試劑以套餐庫存爲準，服務開始後，服務費將按全套服務套餐價格計算。我們承諾以合理、高效、優質的方式提供服務。

1. Congratulations to the publication of the scRNAseq work by Prof. Ko Wing-Hung titled ‘Single-Cell RNA Sequencing Reveals Heterogeneity of ALI Model and Epithelial Cell Alterations after Exposure to Electronic Cigarette Aerosol’ on Heliyon (IF:3.4)
2. Congratulations to the publication of the scRNAseq work by Prof. Alfred Cheng titled ‘Targeting PPAR-gamma counteracts tumour adaptation to immune-checkpoint blockade in hepatocellular carcinoma’ on Gut (IF: 31.795)
3. Congratulations to the publication of the research work using our service by Prof. Jun Yu from CUHK Institute of Digestive Disease titled ‘Targeting m6A reader YTHDF1 augments antitumour immunity and boosts anti-PD-1 efficacy in colorectal cancer’ on Gut (IF: 31.795)
4. Congratulations to the publication of the research work using our service by Prof. Jun Yu from CUHK Institute of Digestive Disease titled ‘Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice’ on Cell (IF: 66.85)
5. Congratulations to the publication of the research work using our service by Prof. Sham Mai Har from SBS titled ‘Ptch1 is essential for cochlear marginal cell differentiation an stria vascular formation’ on Cell Reports (IF: 9.995)
6. Congratulations to the publication of the scMultiome (RNA + ATAC) work by Prof. Alfred Cheng titled ‘Pharmacological activation of STAT1-GSDME pyroptotic circuitry reinforces epigenetic immunotherapy for hepatocellular carcinoma ’ on Gut (IF: 31.795)

1. Congratulations to the publication of the research work using our service by Prof. Jun Yu from CUHK Institute of Digestive Disease titled ‘Targeting m6A reader YTHDF1 augments antitumour immunity and boosts anti-PD-1 efficacy in colorectal cancer’ on Gut (IF: 31.795)
2. Congratulations to the publication of the research work using our service by Prof. Jun Yu from CUHK Institute of Digestive Disease titled ‘Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice’ on Cell (IF: 66.85)
3. Congratulations to the publication of the research work using our service by Prof. Sham Mai Har from SBS titled ‘Ptch1 is essential for cochlear marginal cell differentiation an stria vascular formation’ on Cell Reports (IF: 9.995)
4. Congratulations to the publication of the scMultiome (RNA + ATAC) work by Prof. Alfred Cheng titled ‘Pharmacological activation of STAT1-GSDME pyroptotic circuitry reinforces epigenetic immunotherapy for hepatocellular carcinoma ’ on Gut (IF: 31.795)

• 10X Genomics – Single Cell Gene Expression
• 10X University – A step-by-step learning and training environment
• 10X Datasets
• 10X Publications
• Worthington Tissue Dissociation Guide